文献类型：期刊文章

作　　者：刘火安[1] 王伯初[1] 戴传云[1] 邵志勇[2] 何从林[1] 贾云[3]

机构地区：[1]重庆大学生物工程学院 [2]Interdepartment of Genetics Iowastate University,Iowa 50011,USA [3]重庆科技学院化学与生物工程学院

出　　处：《Journal of Chinese Pharmaceutical Sciences》

基　　金：Science and Technology Committee of Chongqing inChina(CSTC.2004BB5122).

年　　份：2006

卷　　号：15

期　　号：2

起止页码：121-126

语　　种：中文

收录情况：CAB、CAS、DOAJ、EMBASE、IC、JST、SCOPUS、ZGKJHX、普通刊

摘　　要：Aim To screen the optimum macroporous resin and conditions for the isolation and purification of flavonoids from Radix Puerariae. Methods The static and dynamic adsorption/desorption methods were used, and the separation and purification process was evaluated by measuring the concentration of total flavonoid in the fractions with UV spectrophotometer. Results The SP70 macroporous resin was the most effective compared with other macroporous resins. The optimum conditions were screened, which were 0.5 g· mL^- 1 corresponding to crude drug for concentration of extract, pH 5 - 6, and appended 60 times the volume of the resin bed （BV） with the adsorption speed 2 BV·h^-1, and the volume of aq. 70% （V/V） ethanol as eluant was 5 BV with desorption speed 2 BV·h^-1. By this method, the final contents of total flavonoids exceeded 80%. Conclusion The SP70 macroporous resin is the most effective one for large-scale isolation and purification of flavonoids from Radix Pueraria, which meets industrial needs.

关 键 词：Radix Puerariae  total flavonoids  macroporous resin  SEPARATION PURIFICATION

分 类 号：R284.2[中药学类]