文献类型：期刊文章

作　　者：赵健元[1,2] 李进华[1,2] 柳杨[1,2] 尹华宝[1,2]

机构地区：[1]安徽大学生命科学学院,合肥230039 [2]安徽省生态工程与生物技术重点实验室

出　　处：《兽类学报》

基　　金：国家自然科学基金资助项目(30370209;39970709;30570237);安徽省教育厅自然科学基金重点项目(2005KJ021ZD);教育部骨干教师资助计划(18557);安徽省优秀青年基金资助项目(04043070);安徽大学211工程学术创新团队资助项目

年　　份：2005

卷　　号：25

期　　号：4

起止页码：410-413

语　　种：中文

收录情况：AJ、BDHX、BDHX2004、BIOSISPREVIEWS、CAS、CSCD、CSCD2011_2012、GEOBASE、IC、JST、PUBMED、SCOPUS、WOS、ZGKJHX、ZR、核心刊

摘　　要：Extracting DNA from faecal sample is a newly developed technology of recent years. Although several methods to extract DNA from faeces have been developed home and abroad, they addressed fresh samples and it is still blank in extracting DNA from old faeces yet. Extracting old faeces’ DNA can make the full use of transfers out or the death animal’s genetic information and expanding the application scope of Molecular Scatology. In this experiment two groups of faeces were launched into DNA extracting. One group had been preserved by absolute ethanol for 3 years and the other group had been preserved by absolute ethanol for half past 4 years. Aiming at the old faecal’s characteristic of small number of cells and high degrading of DNA, several repetitions(4 tubes) were carried out at the same time in this experiment. Then 4 different DNA extracting methods (Preprocess-phenol/chloroform extraction method, Guanidine thiocyanate method, CTAB method, QIAamp DNA Stool Mini Kit, Qiagen) were followed. From these parallel methods and their corresponding results, we could get the best method while the possibility of extracting DNA from old faecal was studied. After DNA was dissolved in TE we got the original DNA. Finally we collected the original DNA from the 4 repetitions then purified and concentrated it by using DNA purification kit. In the end, the results of PCR amplification shows that only Preprocess-phenol/chloroform extraction method can extract enough DNA for PCR from two groups of faeces. In our study, we extracted DNA from old faeces successfully and got some genetic information from mtDNA. This result confirms the possibility of putting old faecal into molecular scatology study.

关 键 词：陈旧粪便  保存  DNA提取 PCR扩增

分 类 号：Q954.53]