文献类型：期刊文章

作　　者：周雪媚[1] 李永清[2] 张培君[2] 王宏俊[2] 佘锐萍[1] 章振华[3] 罗长保[4]

机构地区：[1]中国农业大学动物医学院,北京100094 [2]北京市农林科学院畜牧兽医研究所,北京100089 [3]江西农业大学动物医学院,南昌330045 [4]广州出入境检疫检验局,广州510623

出　　处：《病毒学报》

基　　金：国家863计划(No;2003AA24112002)

年　　份：2005

卷　　号：21

期　　号：6

起止页码：474-477

语　　种：中文

收录情况：BDHX、BDHX2004、BIOSISPREVIEWS、CAB、CAS、CSCD、CSCD2011_2012、IC、JST、PUBMED、RCCSE、SCOPUS、WOS、ZGKJHX、核心刊

摘　　要：In this study,a 1.35 kb DNA fragment encoding the VP2 protein of infectious bursal disease CJ801 isolate was obtained by PCR and the sequence was determined in T vector.The VP2 gene was cloned into the vector of pcDNATM4/His/LacZ,then the expression cassette including the VP2 gene of IBDV and LacZ gene of E.coli controlled by CMV promoter were inserted into US2 gene of MDV CVI988/Rispens to give a transfer vector of pUS2-VP2.The complex of pUS2-VP2 and DOTAP was transfected into MDV infected CEF.The recombinant MDV expressing LacZ gene were selected and purified on 96-well plate by blue plague.The complete VP2 gene inserted into MDV was detected by PCR.Western blot and immunostaining results demonstrated that VP2 protein of IBDV was expressed by recombinant MDV.

关 键 词：传染性法氏囊病病毒 VP2基因 重组马立克氏病毒  

分 类 号：S858.31]