文献类型：期刊文章

作　　者：刘海萍[1] 王兴明[1] 戴亚堂[1] 宋绵新[1]

机构地区：[1]西南科技大学材料科学与工程学院应用化学研究所,绵阳621010

出　　处：《无机化学学报》

基　　金：国家自然科学基金资助项目(No.40072020);四川省教育厅自然科学基金重点资助项目(No.2000-A56)

年　　份：2005

卷　　号：21

期　　号：9

起止页码：1422-1426

语　　种：中文

收录情况：BDHX、BDHX2004、CAS、CSCD、CSCD2011_2012、IC、JST、RCCSE、RSC、SCI(收录号：WOS:000232790100031)、SCI-EXPANDED(收录号：WOS:000232790100031)、SCIE、SCOPUS、WOS、ZGKJHX、核心刊

摘　　要：The interaction of Methylthymol Blue(MTB)-Zinc! compound and Alizarin Red S(ARS)-Aluminum" compound with Bovine serum albumin (BSA) was investigated by UV-Vis spectrophotometric method in acidic buffer solution. MTB-Zn!-BSA was a blue color compound, which possesses maximum absorption at 613 nm with 172 nm, 174 nm and 18 nm of red shift compared to the MTB, MTB-BSA and MTB-Zn! complexes respectively. Dual wavelength substantial amount ratio method, balance dialysis substantial amount ratio method and unity wavelength substantial amount ratio method were compared. The following results were obtained: the apparent molar absorptivity of MTB-Zn! with BSA was ε=2.20×104 L·mol-1·cm-1. Conditional proportion were defined, nMTB∶nZn!∶nBSA=2∶2∶1; condition combination constant, K=2.07×1010. Combination proportion were defined, nARS∶nAl"∶ nBSA=6∶4∶1. Condition equilibrium constant of reaction of ARS-Al" with BSA was K=8.80×108. The apparent mo- lar absorptivity of ε=2.65×104 L·mol-·1cm-1. It is suggested that combination between BSA and MTB-Zn" is due to coordination force. That combination between BSA and ARS-Al" is due to the coordination bond and electro- static force.

关 键 词：UV-Vis光谱法  甲基百里酚蓝-Zn(Ⅱ)-配合物  牛血清白蛋白 茜素红S-Al(Ⅲ)-配合物  

分 类 号：O614.124.1] O614.131[化学类]