文献类型：期刊文章

作　　者：李运涛[1] 李加纳[1] 柴友荣[1] 杨成军[1] 雷波[1]

机构地区：[1]西南农业大学农学与生命科学学院,农业部生物技术与作物品质改良重点实验室,重庆市油菜工程技术研究中心,重庆400716

出　　处：《分子植物育种》

年　　份：2005

卷　　号：3

期　　号：4

起止页码：485-492

语　　种：中文

收录情况：CAB、CAS、CSCD、CSCD_E2011_2012、JST、RCCSE、ZGKJHX、普通刊

摘　　要：By using RACE (rapid amplification of cDNA ends) based homologous cloning strategy, we have successfully isolated the genomic and full-length cDNA sequences of a gene encoding typical DFR (dihydroflavonol-4-reductase) from black-seeded Brassica campestris L. var. oleifera DC.. The gene, designated BcDFR here, is 1 722bp in length and harbors 5 introns with typical splice sites of plant DFR genes. BcDFR cDNA is 1 311bp in length with a 1 158bp ORF as well as a 25bp 5’ UTR and a 128bp 3’ UTR. The encoded BcDFR protein is 385 aa with a calculated Mw of 42.85kD and a pI value of 5.55. The nucleotide and amino acid sequences of this gene share extensive homologies to plant DFR genes of wide origins especially high similarities to Cruciferous DFR genes. Sequence analyses such as phylogenetic analysis, conserved domain search and substrate specificity region detection all indicated that BcDFR gene is a quite potentially biofunctional gene. Its cloning enables us to further dissect the possible relatedness between DFR gene and Brassica seed coat color traits and to create transgenic novel yellow-seeded rapeseed germplasm through antisense- or RNAi-suppression of DFR gene expression in black-seeded elite cultivars.

关 键 词：白菜型油菜 序列分析  R基因  克隆

分 类 号：S565.4] Q78]