文献类型：期刊文章

作　　者：关伟军[1] 马月辉[1] 丁鸿[2] 于太永[3] 张海艳[4] 梁海青[5]

机构地区：[1]中国农业科学院畜牧研究所,北京100094 [2]广东揭阳职业技术师范学院,广州522000 [3]西北农林科技大学动物科技学院,杨凌712100 [4]洛阳师范学院生命科学系,洛阳471022 [5]山西农业大学动物科技学院,太谷030801

出　　处：《畜牧兽医学报》

基　　金：国家自然科学基金(30100132);基础性工作重大专项(2001DEA10006)

年　　份：2005

卷　　号：36

期　　号：5

起止页码：511-516

语　　种：中文

收录情况：BDHX、BDHX2004、CAB、CAS、CSA、CSCD、CSCD2011_2012、DOAJ、EMBASE、IC、JST、PROQUEST、RCCSE、WOS、ZGKJHX、ZR、核心刊

摘　　要：Taking Small Tail Han Sheeps ear margin as the study materials, we succeeded in establishing a fibroblast cell bank which content of samples was 52 by the method of explant culture directly and cryopreservation technique. The biological analysis results showed that population doubling time (PDT) of cells was approximately 24 h, diploid account for 90%～96% in chromosome. Confirmed by isoenzyme analysis,this cell line had no cross-contamination.The test results of the bacterium, fungi, virus, mycoplasma were negative. The transfection rate was high, and the extro-gene can be effectively expressed. Every indexes of the cell bank met all the standard quality controls of ATCC. It had not only preserved the germplasm resources of this important Small Tail Han Sheep on the cell level but also provided valuable material for the research of genome, postgenome and somacloning. Meanwhile, the setting-up of this technological platform will offer technology and theory for storing the cell genetic resources of other animals and poultry.

关 键 词：成纤维细胞系 特性研究  小尾寒羊 生物学  1999年  1995年  组织  资源危机 畜禽品种 世界范围  信息调查 地方品种  灭绝  专门化  濒危 比例  种质  遗传  

分 类 号：S814.8] S826]