文献类型：期刊文章

作　　者：汪开毓[1] 赵德明[2] 耿毅[1] 黄小丽[1] 刘光迅[3]

机构地区：[1]四川农业大学水产系,四川雅安625014 [2]中国农业大学动物医学院,北京100094 [3]四川省水产研究所,四川成都617731

出　　处：《水产学报》

基　　金：四川省学术带头人后备人选资助基金

年　　份：2004

卷　　号：28

期　　号：6

起止页码：733-737

语　　种：中文

收录情况：AJ、BDHX、BDHX2000、CAB、CAS、CSA、CSA-PROQEUST、CSCD、CSCD2011_2012、IC、JST、PROQUEST、RCCSE、SCOPUS、WOS、ZGKJHX、ZR、核心刊

摘　　要：Hepatic cells apoptosis induced by olaquindox in common carp, Cyprinus carpio, were studied. The test was conducted for 30 days, with dose of 200mg·kg-1 feed. Terminal deoxynucleotidy transferase mediated dUTP-Biotin nick-end labeling （TUNEL）, electronic microscopic and flow cytometry were performed to analyze apoptosis. The result showed that hepatic cells apoptosis were indentified under light microscope with TUNEL staining; chromatin condensation, chromosal clumping and margination, and apoptotic bodies were observed under electronic microscope; and sub-G1 PEAK （AP peak） in hepatic cells could be detected by flow cytometric method. The apoptotic rate measured by flow cytomtry increased with dye-poison time, on the 7th,14th,20th,25th and 30th day, the apoptotic rate was 2.05%, 6.25%, 9.49%, 13.31% and 17.44% respectively.

关 键 词：鲤  细胞凋亡 喹乙醇 肝细胞

分 类 号：S941.91]