文献类型：期刊文章

作　　者：伍翠芳[1] 顾继锐[1] 刘会明[2] 张文露[2] 洪芳[2] 蒋天明[1] 吴江[1] 徐恒[2]

机构地区：[1]成都通威水产科技有限公司,成都610081 [2]四川大学生命科学学院,成都610064

出　　处：《四川大学学报（自然科学版）》

基　　金：国家水利部"948"项目;通威集团科技发展项目

年　　份：2004

卷　　号：41

期　　号：5

起止页码：1085-1087

语　　种：中文

收录情况：BDHX、BDHX2000、BIOSISPREVIEWS、CAS、CSCD、CSCD2011_2012、JST、MR、RCCSE、ZGKJHX、ZMATH、ZR、核心刊

摘　　要：The recombinant plasmid pFV2cfGH was constructed using Growth hormone gene which was cloned from the channel catfish cDNA library,the β-action promoter of carp and the polyadenylation signal from the salmon growth hormone gene, and recombinant plasmid pFV2cfGH was microinjected into the fertilized eggs of the colorful crucian carp. 60 transgenic Colorful Crucian carps total DNA were got ,and growth hormone gene were detected by PCR. The result shows that the channel catfish growth hormone gene had been transmitted into the genome of the Colorful Crucian carp and the integration rate is about 47%.

关 键 词：生长激素基因 转基因鱼 观赏鱼类  生长速度  转基因育种技术 转基因方法  基因安全 哺乳动物  体形  分子生物学研究  

分 类 号：N02] Q78]