文献类型：期刊文章

作　　者：王兴明[1] 董发勤[1] 丁立生[2] 彭汝芳[1] 廖辉伟[1]

机构地区：[1]西南科技大学化学与生物工程系,绵阳621002 [2]中国科学院成都生物研究所,成都610041

出　　处：《无机化学学报》

基　　金：国家自然科学基金资助项目(No.40072020);四川省教育厅自然科学基金重点资助项目(No.2000鄄A56)。

年　　份：2004

卷　　号：20

期　　号：8

起止页码：920-924

语　　种：中文

收录情况：BDHX、BDHX2000、CAS、CSCD、CSCD2011_2012、IC、JST、RCCSE、RSC、SCIE、SCOPUS、WOS、ZGKJHX、核心刊

摘　　要：The interaction of Zincon(ZCN)-Cu(Ⅱ) metal complexe with bovine serum albumin (BSA) was investigated by UV-Vis spectrophotometric method in acidic buffer solution (pH=4.25). ZCN-Cu(Ⅱ)-BSA was a blueness color coordination compound, with maximum absorption at 605 nm with 10 nm, 60 nm and 140 nm of red shift compared to the ZCN-Cu(Ⅱ) complex, ZCN-BSA complex and ZCN. According to molar ratio method and dual wavelength method, the apparent molar absorptivity of ε was equal to 4.82×105 L·mol-1·cm-1. Conditional constants were defined as ZCN∶Cu(Ⅱ)∶BSA=10∶5∶2. Condition combination constant of ZCN-Cu(Ⅱ) and BSA K was 8.82×104. A combined mechanism was suggested between BSA and ZCN-Cu(Ⅱ) by electrostatic force (Primary action force) and distant water force. Position of Impact might be arginine, proline, histidine, tyrosine and cystine. Contemporary formation of electron transfer complexes is an important cause in forming ZCN-Cu(Ⅱ)-BSA complexes.

关 键 词：蛋白质  锌试剂-铜配合物  牛血清蛋白 反应机理

分 类 号：Q512.1]