文献类型：期刊文章

作　　者：李菁[1] 林彤[1] 高闪电[2] 丛国正[2] 独军政[3] 邵军军[1] 常惠芸[1]

机构地区：[1]中国农业科学院兰州兽医研究所国家口蹄疫参考实验室,甘肃兰州730046 [2]中国农业科学院兰州兽医研究所农业部畜禽病毒学重点开放实验室,甘肃兰州730046 [3]中国农业科学院兰州兽医研究所家畜疾病病原生物学国家重点开放实验室,甘肃兰州730046

出　　处：《Agricultural Science & Technology》

基　　金：Supported by National Supporting Plan(2006BAD06A14);Transgenic Major Projects(2008ZX08011-004)~~

年　　份：2010

卷　　号：11

期　　号：2

起止页码：23-26

语　　种：中文

收录情况：CAB、CAS、CSA、CSA-PROQEUST、PROQUEST、普通刊

摘　　要：[Objective] The research aimed to induce the expression of FMDV structural protein VP1 in E.coli and purify the protein,then detect the activity.[Method] The fragment coding VP1 was amplified by PCR and doubly digested with BamH Ⅰ and XhoⅠ,then cloned into expression vector pGEX-4T-1 and pPROExHTb respectively to get recombinant plasmid pGEX-4T-1-VP1 and pPROExHTb-VP1.The recombinant plasmid pGEX-4T-1-VP1 and pPROExHTb-VP1 was transformed into E.coli BL21(DE3)and induced by IPTG,fusion protein was identifie...

关 键 词：A-type FMDV  Structural protein VP1  Expression and purification  

分 类 号：S852.65]